The measured levels were indexed using the following equation: Cut-off index (COI) = ( sample− NC) / ( PC− NC), where PC = positive control and NC = negative control. Serum WFA +-M2BP level was measured using a lectin-antibody sandwich immunoassay using a chemiluminescence enzyme immunoassay machine (HISCL-5000, Sysmex, Kobe, Japan). Consecutive adult NAFLD patients who had a liver biopsy were included. Wisteria floribunda agglutinin-positive Mac-2 binding protein (WFA +-M2BP) has been suggested to be useful for the assessment of disease severity in non-alcoholic fatty liver disease (NAFLD).
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